Downloads#

Note

Rows with an entry in the Segmentation/Spots column mean that download contains the pre-computed segmentation labels and/or spot detection coordinates for that dataset.

Course Data#

03 - Introduction to Digital Images

ItemDatasetSegmentation/SpotsNotes
Working with Bioimages Working with Bioimages Dataset

04 - Image Segmentation

ItemDatasetSegmentation/SpotsNotes
Classical Methods Segmentation and Spot Detection Dataset Segmentation and Spot Detection ResultsGenerated from CCDB_6843
Machine Learning Methods - Ilastik Pixel Classification Ilastik Pixel Classification Dataset Ilastik Pixel Classification ResultsFrom Ilastik Data Mitocheck 2D+t
Deep Learning Methods - Cellpose Segmentation and Spot Detection Dataset Segmentation and Spot Detection ResultsSame dataset as `Classical Methods`, generated from CCDB_6843
Deep Learning Methods - Cellpose 3D Cellpose 3D DatasetFrom BBBC033
Deep Learning Methods - Cellpose Training Dataset Cellpose Training DatasetFrom Cellpose Github Repository

05 - Spot Detection

ItemDatasetSegmentation/SpotsNotes
Spotiflow Segmentation and Spot Detection Dataset Segmentation and Spot Detection ResultsSame dataset as `Classical Methods` and `Cellpose`, generated from CCDB_6843
Spotiflow – Finetuning Dataset Spotiflow Finetuning DatasetFrom Spotiflow example notebook, MERFISH dataset from Zhang et al, 2021

06 - Measurement & Quantification

ItemDatasetSegmentation/SpotsNotes
Measurement Pipeline Segmentation and Spot Detection Dataset Segmentation and Spot Detection ResultsSame dataset as `Classical Methods` and `Cellpose`, generated from CCDB_6843
Nested Measurements Group Work Dataset(included in dataset)
Background Correction Background Correction Dataset

07 - Colocalization

ItemDatasetSegmentation/SpotsNotes
Pixel Intensity Based Manders & Pearson's Colocalization Dataset
Object Based Object Based Colocalization Dataset
Spatial Statistics Segmentation and Spot Detection Dataset Segmentation and Spot Detection ResultsSame dataset as `Classical Methods`, `Cellpose` and `Spotiflow`, generated from CCDB_6843

BoBiac Exercises#

ItemDatasetSegmentation/SpotsNotes
Group Work 1: Building your Own Segmentation and Spot Detection Pipeline Group Work 1 Dataset(included in dataset)
Group Work 2: Analysing Oscillations in Time-Series Images Group Work 2 Dataset(included in dataset)
Group Work 3: Colocalization Analysis Segmentation and Spot Detection Dataset Segmentation and Spot Detection ResultsUse the results from `Classical Methods`, `Cellpose` and `Spotiflow` notebooks if you have them. Same dataset as `Classical Methods`, `Cellpose` and `Spotiflow`, generated from CCDB_6843

Download All Course Materials#